The Nanopore Site

PhD position in the Sabina Caneva group at Delft: 

We are looking for a highly motivated and enthusiastic PhD student to work in the field of mechanobiology. The interdisciplinary project concerns the development of a nanoelectromechanical system that integrates surface acoustic wave (SAW) and nanopore technologies to mechanically manipulate biological molecules and materials in biomimetic membranes.
Sensing mechanical cues is crucial for many biological processes. Mechanically-activated protein ion channels detect blood flow, lung inflation and sound waves and are responsible for the regulation of heart beating, breathing and hearing. These protein nanopores respond to pressure by enabling or blocking ion transport across biological membranes. Malfunctioning of these channels accounts for dozens of human diseases. To understand the nanomechanical opening/closing mechanisms of these proteins, it is essential to use tools that can track their dynamic response in real time.
You will create an experimental platform that interfaces acoustic tweezers with high-resolution ionic current detection to study the effect of SAW stimulation on the localization and actuation of force-sensitive protein nanopores. Using fluorescence microscopy and single-molecule conductance measurements, you will (i) monitor the spatial and temporal organization of biomolecules on the nanofluidic chip and (ii) measure the ionic current across the membrane to detect conformational changes induced by membrane perturbation in real-time. In addition, by creating mimics of normal and stiffer membranes (e.g. tumor tissue), you will investigate how the mechanical properties of the membrane influence ion transport through the nanopore.
Results from the project will, on one hand, yield fundamental knowledge of biomechanics at the single-molecule level, and on the other, they will form the basis for a novel lab-on-chip device that enables screening of compounds that inhibit or enhance transmembrane protein function.

More details:
https://www.academictransfer.com/en/291532/phd-acoustic-actuation-of-force-sensitive-protein-nanopores/

​We seek a highly motivated biophysics/bioengineering postdoctoral fellow to join our interdisciplinary computational and experimental group which is engaged in the DARPA ECHO project, the MoTrPAC consortium and other large-scale programs.
 
The fellow will study device development for nanopore epigenetic assays and other biophysical assays, with opportunities for publication, patenting and commercial development.
 
The successful candidate should have a PhD in biophysics, bioengineering, chemistry, physics, engineering, or a closely related discipline and experience with nanopore technology with sequencing (solid-state and protein pores for applications in nucleic acids and proteins).
 
Experience with Oxford Nanopore, micro- and nano- fluidics; molecular and cellular biology; semiconductor processing; MEMS; micro- and nano- devices; low-noise, phase-sensitive lock-in measurements; programming in MATLAB and LABVIEW and working knowledge of biophysical and bio-characterization techniques (such as AFM, HPLC, LC-MS and functional assays) are desirable.
 
Please send a CV and the contact info for 3 referees to angelo.gaitas@mssm.edu
 
https://careers.mountsinai.org/jobs/2523250?lang=en-us
 

 Iridia is an emerging San Diego company bringing together DNA chemistry,electronics, molecular biology and nanofabrication technology to develop arevolutionary long-term data storage platform.
The Iridia Research Team is looking for a nanopore scientist with experienceand expertise in the field of biophysics and solid-state nanopores. This is arare and exciting opportunity to apply your knowledge and abilities to thedevelopment of a transformational technology.
 
Job Summary:
We are seeking a proven self-starter that can perform independent, wellcontrolledresearch as part of a multi-disciplinary technology developmentteam, with a focus on the use and modeling of solid-state nanopores for detection and interpretation of DNA translocations. Your work will be integral to the design and optimization of the world’s first integrated DNAbased data storage microchip.
 
Tasks and responsibilities:
• Design, conduct, trouble-shoot and analyze solid-state nanopore DNA translocation experiments.
• Assist with modeling of solid-state nanopore DNA translocation events.
• Contribute to the design of microfluidic devices with integrated solid-state
nanopores.
• Assist with the integration of molecular biology protocols into microfluidic
devices.
• Maintain records and prepare presentations for providing technical
updates, data analysis, conclusions and recommendations.
• Lead collaborations with 3rd parties in industry and/or academia.
• The successful candidate will be self-motivated, dedicated, and enthusiastic, with the ability to effectively communicate with scientists from different disciplines. The successful candidate should have a passion for scientific excellence, a committed work ethic, and an assertive but respectful personality.
 
Preferred experiential background:
• Use of solid-state nanopores for the study of DNA &/or protein translocations.
• Modeling of pore characteristics and DNA translocations through solidstate nanopores.
• Fabrication and characterization of solid-state nanopores.
• Surface chemistry and science, surface modification and metrology.
• Expertise in microfluidics and electrochemistry is highly desirable.
• Industry experience is desired, but not required.
• Experience in building, testing & troubleshooting analog circuits.
• Background in physics, electronics, digital signal processing, statistics,
programming, algorithm implementation.

Preferred educational background:
• A Ph.D. in Physics (Biophysics) or related discipline.
• Post-doctoral experience desired, but not required.
In addition to exciting science and a positive work culture, Iridia offers an attractive compensation package which includes stock options, generous vacation time, plus family health and medical benefits.
​
Iridia Inc., is proud to be an EEO Employer
 
 

​Position Title: Postdoc Researcher (Multiple positions)

Location: Nanjing, China 

Salary: ¥300k (~£34k), depending on experience, with a highly competitive bonus 

Additional Benefits:

1. Eligible to apply as a principal investigator (PI) for research grant, e.g. NSFC;
2. Eligible to apply for the “Global Postdoc Exchange Scheme”.

Research Group Description:
After re-joining Nanjing University at the end of 2018, I am now establishing a new research group which focuses on the development of novel electrochemical measurement methods to reveal the characteristics and dynamics of single entities, as well as the design and application of single molecule interface. Research areas include, but are not limited to, nanopore single molecule electroanalysis, biointerface spectroelectrochemistry and integrated biosensors. For more information of Yi-Tao Long’s group, please visit http://hysz.nju.edu.cn/ytlong/. 

Job Requirements:

1. Have a strong desire to work in academia (compulsory);
2. Received comprehensive research training from top universities (compulsory);
3. Demonstrated a high-level of skill in teamworking, communication, and academic writing (compulsory);
4. Previous research experiences in any of the following subjects: electrochemistry, optics, biology, nanotechnology, electrical engineering, artificial intelligence. (optional)

Primary Job Duties: 
Design and perform experiments, analyze data, write papers, deliver presentations, etc.

Job Requirements:

• Degree in a biology or engineering field such as Biophysics, Bioengineering, Molecular Biology, Computational Biology, or Biomedical Science (MS or higher).
• Experience with electrophysiology or nanopores (preferable nanopore-based single molecular detection) is strongly preferred.
• Computer programming skillsets are a plus (python, matlab, labview).

Contact: yitaolong@nju.edu.cn
 

We are looking for a postdoctoral researcher to join the Nano-biotechnology group at the IBM T.J. Watson Research Center. The candidate should have proven experience in theoretical and experimental biophysics including continuum. See further at https://www.linkedin.com/jobs2/view/17710466